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EFFECT OF PEA SEED PRETREATMENT WITH MELATONIN AND GERMINATION SUBSTRATES ON RESPIRATORY ACTIVITY AND EMBRYO GROWTH AFTER SHORT-TERM DEHYDRATION OF SWELLING SEEDS

Table 1 - Effect of seed dehydration and melatonin treatment on embryo respiration of group 1 (1) and group 2 (2) of 18-hour-old pea seeds grown on water or Hoagland 1/2 medium

Germination medium

Respiration rate (nmol O/(min g dry w))

Water (group 1)

Hoagland's medium (group 2)

Variant / respiration pathway

Vt

Vcyt

Valt

Vres

Vt

Vcyt

Valt

Vres

Control

5806 ± 170 a

3574 ± 170 h

1562 ± 125 m 

671± 150 t 

5869 ± 160 a 

3304 ± 150 i

917 ± 140 n  

1648± 140 y  

+ melatonin

4515 ±150 b

3347 ± 150 h

291 ± 120 o  

873 ± 140 x  

6881 ± 150 e   

4583 ± 150 j  

557 ±150 p  

1741 ± 140 y  

Dehydration

7275 ± 210 c

4710 ± 150 b  

1070 ± 120 r  

1495 ± 130 z  

4161 ± 150 f  

2602 ± 150 k  

1214 ± 130 r  

346 ± 110 z-1   

Dehydration + melatonin

3769 ± 150 d

2734 ± 140 k  

281 ± 140 s  

754 ± 130 z-3   

8675 ±160 g   

5398 ± 130 l  

1158 ± 140 r  

2120 ± 140 z-2   

the table shows the arithmetic mean values and their standard errors. Statistically significant differences between mean values were determined using Tukey test in ANOVA programme, results marked with different letters are statistically significant at P ≤ 0.05.Water deficit of embryo tissues germinated on water or Hogland's medium ½ (%)