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VETERINARY SCIENCE

ISSN 2227-6017 (ONLINE), ISSN 2303-9868 (PRINT), DOI: 10.18454/IRJ.2227-6017
ПИ № ФС 77 - 51217, 18+

SELECTING GENES FOR SALMONELLA ENTERITIDIS IDENTIFICATION

Posted in 2018, Issue №10(76) October 2018, VETERINARY SCIENCE | 0 comments

Currently, polymerase chain reaction method (PCR) is one of the most widely used methods of laboratory diagnosis of salmonellosis. This paper shows the possibility of using PCR in combination with the sequencing method to detect and subsequently identify Salmonella Enteritidis. The sefA gene was selected as a marker gene for analysis after an analytical review. This field contains areas with a high degree of conservatism, which is a prerequisite for selecting primers for PCR. The proposed primers ensure the identification of Salmonella Enteritidis in biological materials within a short period.

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RABBIT PARASITOPHAUNA MONITORING IN SMOLENSK DISTRICT OF SMOLENSK REGION, TREATMENT OF MOST DISTRIBUTED DISEASES WITH NEW DOMESTIC COMPLEX PREPARATION – APRUMET

Posted in 2018, Issue №10(76) October 2018, VETERINARY SCIENCE | 0 comments

A current parasitological situation among rabbits of various breeds kept in private farms of the Smolensk region was monitored in this work, the most common types of parasites were identified. Rabbits were treated with a new domestic, unequalled, complex antiparasitic drug – Aprumvet, which includes the coccidiostatic amprolium and the nematoxidin component – alben substance. As a result, positive effect was obtained, namely the treatment was quite effective. It was also found that there is no negative effect of the drug on the animal’s body, this was confirmed by previous studies.

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EFFICIENCY OF IMMUNOMODULATOR APPLICATION FOR PREVENTION OF PARAINFLUENZA-3 IN CALVES

Posted in 2018, Issue №10(76) October 2018, VETERINARY SCIENCE | 0 comments

Since 20-25% of calves have immuno-deficiency in one or another link, immunological prophylaxis including the one against parainfluenza-3 (PG-3) is not always effective. The use of “polyoxidonium vet solution” immunomodulator allows increasing the titers of specific antibodies against PI-3 when vaccinating calves with the inactivated “Kombovac-R” vaccine, on average, three times, compared to the control group vaccinated without the use of an immunomodulator. Two months after the revaccination in the control group, the disease of PI-3 calves was registered in 5% of the animals in the experimental group and there were no cases of PI-3 among calves. The use of “polyoxidonium vet solution” had a positive effect on the phagocytic activity of neutrophils, normalized white blood values and the ratio of T and B lymphocytes. Thus, the use of “polyoxidonium vet solution” immunomodulator helps to increase the effectiveness of preventive measures in case of PI-3.

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CLINICAL EXPERIENCE OF APPLICATION OF MORENIT FOR PREVENTION OF HEPATOSIS IN LARGE CATTLE

Posted in 2018, Issue №8(74) August 2018, VETERINARY SCIENCE | 0 comments

The article presents the results of studying the prophylactic properties of the Morenit drug in hepatosis in cattle. The use of a complex preparation based on bentonite clay at the rate of 3% for the dry substance of feed to high-yielding cows of the Holstein-Friesian breed for 4 weeks ensures the prevention of hepatopathies in dairy cattle, has an optimizing effect on the metabolism of animals, activates the reparative processes of the hepatic tissue and improves its functioning, and also reduces endogenous intoxication of the body.

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VIRULENT AND REPRODUCTIVE PROPERTIES OF ATTENUATED EIMERIA ACERVULINA STRAIN

Posted in 2018, Issue №7(73) July 2018, VETERINARY SCIENCE | 0 comments

The Eimeria acervulinа L-2-15 strain is part of a live attenuated vaccine for the prevention of chicken eimeriosis. The strain was obtained by the selection of the field isolate of the eymeriosis according to the shortened prepatent period by a series of passages through a susceptible bird. The paper compares the virulent and reproductive properties of the vaccine strain after a series of passages on broiler chickens. The result of the study is the stability of the reproductive and virulent properties of the Eimeria acervulinа L-2-15 strain of the first and sixth passages through the organism of a susceptible bird. High infectious doses of the parasite do not cause the death of broiler chickens, but significantly reduce the increase in body weight.

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SPREADING OF ZOOANTROPONOZE HELMINTHIASES OF WILD ANIMALS ON TRANSBAIKAL TERRITORY

Posted in 2018, Issue №1(67) January 2018, VETERINARY SCIENCE | 0 comments

The authors studied the epizootic situation in zooantroponoze helminthiases of wild animals on the Trans-Baikal Territory. There were revealed 15 zooanthroponous helminthiases: trichinosis, spiroccercosis (2 species), fascioliasis, dicrocoliosis, cysticercosis, echinococcosis, dipylidiosis, diphyllobothriasis, dyrofilariasis, toxocarosis, toxaxaridosis, trichostrongyloidosis (2 species) and cranosomatosis.

The obtained data on zooantroponoze helminthiases of wild animals in the Transbaikalian region made it possible to assess the epizootic situation of these diseases and can be used in drawing up plans for the control and prevention of parasitic diseases of domestic animals and humans. The “Map of the spreading of zooanthroponotic helminthiases of wild animals on the Trans-Baikal Territory” was compiled based on the data obtained.

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DISSEMINATION OF OSTEOARTICULAR PATHOLOGIES OF SHEEP IN TRANSBAIKAL TERRITORY

Posted in 2018, Issue №1(67) January 2018, VETERINARY SCIENCE | 0 comments

Osteoarticular pathologies include a large group of various non-contagious diseases, manifested mainly by changes in the osseous system and a number of accumulated secondary features.

The morbidity of alimentary osteodystrophy of sheep is 3.2-4.3% of the number of animals studied in the sheep breeding areas of the Trans-Baikal Territory, and in unfortunate (arid and lean) years the disease becomes a global problem and causes a significant damage. Since the main reason for the development of osteodystrophy in sheep in the Transbaikalian Territory is an alimentary factor, it is necessary to improve the forage base in farms by increasing the sowing of leguminous crops, corn, grass mixtures with leguminous plants rich in high-grade proteins.

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MORPHOLOGY OF CYSTOUROLITHS OF DOGS AND CATS IN ABAKAN

Posted in 2018, Issue №1(67) January 2018, VETERINARY SCIENCE | 0 comments

The amount, size, weight, shape, color and the surface nature of urinary stones extracted from gall bladders and urethras of dogs and cats in the city of Abakan of the Republic of Khakassia were determined with the help of macro- and micromorphological methods. It was found that the uroliths of female dogs and cats differ in large dimensions and in quantitative characteristics. Morphologically, all uroliths of dogs and cats can be divided into the following types: drusoid, spherulitic, combined, smoothed and dendritic. Those uroliths extracted from gall bladders of female dogs, often have a smoothed morphology, and those of male dogs shave smoothed and combined shapes with an equal frequency. There are uroliths of drusoid, spherulitic, combined morphology, and less often – with a smooth morphology in female cats and male cats with equal frequency. Based only on the morphology of the uroliths, it is often impossible to determine reliably their chemical composition.

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CHEMICAL UROLITS COMPOSITION OF DOGS AND CATS IN ABAKAN CITY OF KHAKAS REPUBLIC

Posted in 2018, Issue №1(67) January 2018, VETERINARY SCIENCE | 0 comments

The phase composition of cystourolites of dogs and cats in the city of Abakan in the Republic of Khakassia, as well as the structural state of minerals and their quantitative ratio, were revealed with the use of X-ray diffractometry and infrared spectrometry methods. Urolites of dogs and cats are mostly represented by struvite and carbonate-hydroxylapatite of varying degrees of crystallinity. Dog urolites in terms of chemical composition showed greater variability compared to those of cats, which is probably due to a more variable habitat and fodder.

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CLINICAL JUDGMENT OF INFLUENCE OF CARPHOPHENUM PHARMACEUTICAL FORM ON GASTROINTESTINAL TRACT OF FINE PET ANIMAL

Posted in 2017, Issue №12(66) December 2017, VETERINARY SCIENCE | 0 comments

Nonsteroidal anti-inflammatory drugs are used in the practice of veterinary medicine for correction of pathological processes, accompanied by inflammation and pain symptoms. This increases the risk of collateral damage related to the gastrointestinal tract of animals. The use of gelatin films of Karprofen allows to reduce the risk of adverse drug event, according to the results of studies, conducted on laboratory animals and clinical trials on dogs.

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FREQUENCY OF OCCURRENCE AND CLINICAL MANIFESTATION OF UROLITHIASIS IN DOMESTIC ANIMALS OF REPUBLIC OF KHAKASIA

Posted in 2017, Issue №12(66) December 2017, VETERINARY SCIENCE | 0 comments

The article examines the revealed pathology of the urinary tract, the frequency of occurrence and clinical manifestation of urolithiasis in dogs and cats of the Republic of Khakassia. Based on the studies, the following conclusions were drawn: the frequency of occurrence of an individual pathology of urinary organs has a distinct specific, sexual and, in some cases, breed predisposition, cystopathology occupies a predominant position over nephropathology, due to a number of factors: resistance of the organism, ways of drifting infection into the organs of urination, stress, conditions of animal welfare. Urolithiasis in dogs and cats in the Republic of Khakassia is registered in 19.5% of cases.

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MAIN FEATURES OF BAYLISASCARIASIS OF NORTH AMERICAN RACOON

Posted in 2017, Issue №12(66) December 2017, VETERINARY SCIENCE | 0 comments

The article contains data on the distribution of baylisascariasis of the North American raccoon. It is noted that the North American raccoon is a species highly infested with baylisascariasis on the territory of the USA and Canada, where the maximum invasive extent reaches 86%. In European countries (Poland), the extent of baylisascariasis infestation in the wild North American raccoon is 3.7%, in Asia (Azerbaijan) it comprises 4.2%. On the territory of Russia, baylisascariasis of the North American raccoon is not recorded. In connection with its high danger for humans, the use of pyrantel embonate, ivermectin, moxidectin, albendazole, febendazole, fludendazole is highly recommended along with the quality control of the performed anthelminthic treatment.

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ORGAN-PRESERVING OPERATIONS IN CASE OF SPLEEN INJURIES OF DOGS

Posted in 2017, Issue №12(66) December 2017, VETERINARY SCIENCE | 0 comments

The aim of the work is to evaluate organ-preserving surgeries on dogs with spleen injuries. According to the results of the research, changes in the rheological properties of blood in the distant postoperative period manifested by the increase in blood viscosity are not expressed after organ-preserving surgeries and are noted in 90% of cases after spleenectomy. After performing organ-preserving surgeries on dogs in the distant postoperative period, there is no disruption of aggregation activity of platelets. Immunity parameters (immunoglobulins Ig G, Ig M, Ig E, phagocytic activity of leukocytes, phagocytic number, phagocytic index) remain within the physiological norm during organ-preserving surgeries on spleen in a remote postoperative period.

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ISOLATED VIABLE SEGMENT OF ANTERIOR ABDOMINAL WALL OF PIG AS A SUBSTITUTE OF THE WHOLE ANIMAL PROVIDING THE EVALUATION OF THE EFFECTIVENESS OF BLEACHER OF BRUISES

Posted in 2017, Issue №11(65) November 2017, VETERINARY SCIENCE | 0 comments

The results of the studies show that it is possible to evaluate the effectiveness of bleacher of bruises in the laboratory without the use of whole animals if isolated segments of the anterior abdominal wall obtained immediately after slaughtering the animals for meat are used for this purpose. It is shown that the injections of animal blood into the skin of isolated segments produced in the laboratory ensure the creation of artificial bruises, and the color dynamics of the skin in the area of such bruises makes it possible to evaluate the effectiveness of bleaching agents. In this regard, the segment of the anterior abdominal wall of a pig with an artificial bruise is proposed as a new biological model that allows screening bleacher of bruises in the laboratory and assessing the local bleaching efficacy of the agents. It is shown that the use of such biological model makes it possible to carry out qualitative pharmacological studies in the absence of live animals and vivarium, so it greatly simplifies and cheapens the process of researching and studying of new drugs.

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ECONOMIC EFFICIENCY OF ANTI-PSOROPTIC ACTIVITIES IN THE SOUTH OF THE TYUMEN REGION

Posted in 2017, Issue №11(65) November 2017, VETERINARY SCIENCE | 0 comments

The paper presents the calculations on the cost-effectiveness of drugs from the group of pyrethroids — veterin and neostomozan and macrocyclic lactones-iversect v and ivomec for the treatment of psoroptic scab of cattle. The economic efficiency of cattle with psoroptic scab per animal is as follows: with veterinary application — 223.86 rubles, iversect v — 224.43 rubles, ivomec puron — 179.26 rubles, neostomozan — 183.07 rubles. The use of 0.1% veterin for the therapy and preventive measures of psoroptic scab in young cattle provides the economic effect of 1 ruble per each 11.63 rubles of expences; 1% of the iversect — 12.8 rubles, ivomek puron — 8.07 rubles, neostomozan in a concentration of 1:400 — 7.98 rubles.

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DISTRIBUTION OF VIRAL DISEASES OF CATS IN TYUMEN CITY

Posted in 2017, Issue №11(65) November 2017, VETERINARY SCIENCE | 0 comments

The results of monitoring the epizootic state of viral diseases of cats in the city of Tyumen for the period from 2012 to 2016 are presented. The infection of cats with panleukopenia, infectious peritonitis, calicivirus and herpesvirus infection, from the total number of animals infected with viral infections is counted; the share of panleukopenia is 55.3%, the infection peritonitis virus infected 33.8% of animals, and the calicivirus and herpesvirus infection infected 6.8% and 4.1% of animals respectively. Seasonal dynamics can be clearly seen in panleukopenia, as cats are more likely to get sick in April, July, and more in October.

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THE EFFICIENCY OF FASTAK IN PSOROPTIC MANGE OF LARGE CATTLE

Posted in 2017, Issue №11(65) November 2017, VETERINARY SCIENCE | 0 comments

During the study period (2014-2016 years) in livestock farms in the south of the Tyumen region psoroptic mange of large cattle was recorded in 24.2±2.7% of the heads, with the predominant lesion of the young (42.4±4.3%). The therapeutic efficacy of the fastak drug in case of psoroptic mange of cattle was studied. FASTAK – 10% solution of cypermethrin, is manufactured by CJSC “Vitar” (Tyumen). Treatment of cattle with 0.5% aqueous emulsion of fastak by spraying from “AutoMax”, twice, with an interval of 7-10 days in a volume of 1.5-2 liters for calves and 2.5-3 liters showed 100% therapeutic efficacy for cattle with psoroptic mange. Fastak in recommended doses and mode of application does not have a pathological effect on the clinical and hematological status of animals.

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CLINICAL AND MORPHOLOGICAL DIAGNOSIS OF THE LYMPHOMA AMONG DOGS

Posted in 2017, Issue № 9(63) September 2017, VETERINARY SCIENCE | 0 comments

It is noted that the cytological method of diagnosis as one of the morphological methods used for verifying lymphomas among dogs is fairly objective. A detailed description of the characteristics of tumour cells in lymphomas with a high degree of malignancy is given. It represents 66.6% of all verified lymphosarcomas among dogs. It is indicated that the proliferation of lymphoid cells with blast morphology is the characteristic feature. Distinctive morphological features including the size and shape of the cells, the configuration and size of the nucleus, the presence of nucleos in the nucleus, the degree of basophilia of the cytoplasm, the presence of cytoplasmic inclusions are indicated. Based on the results obtained, morphological diagnosis of the multi-centric form of lymphomas among dogs of high degree of malignancy is developed. A description of the cytological picture and morphological features of the cells are given, such as polymorphism and nucleolar activity.

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COMPARATIVE CHARACTERISTICS OF TREATMENT METHODS OF ANIMALS WITH THERMAL BURNS

Posted in 2017, Issue № 8(62) August 2017, VETERINARY SCIENCE | 0 comments

Thermal burns are still one of the most common types of injuries, both for small unproductive animals and for large agricultural ones. The relevance of the presented work is that it systematizes and summarizes available information on the therapy of burn disease, the ways of providing first aid and modern innovations on this issue. The most common cause of burns is the effect of hot liquids onto the skin. Less often, animals get burned during fires or as a result of deliberate people’s actions.

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ANALYSIS OF CERTAIN MORPHOLOGICAL CHANGES OF MATURE CATS’ SPLEEN IN MACRO- AND MICROSCOPIC ASPECT

Posted in 2017, Issue № 7(61) July 2017, VETERINARY SCIENCE | 0 comments

The article describes the morphology of the spleen of cats aged 7-16 years. As a result of the work, it was established that cats’ spleen has a certain topography that does not change during ontogenesis. Cats’ spleen has a ribbon-like shape with a narrow dorsal and a wide ventral end. Its colour is dark cherry; the capsule is normally shiny and smooth. Its parietal surface is smooth, too. There are the gates of the spleen on the visceral surface. Spleen is relatively loosely associated with a large omentum in the area of the gate. Spleen is always attached to the left side of the abdominal wall. Its position largely depends on the filling of the stomach and its functional state. In addition, the parameters of the measurements and mass of this organ are given.

Special emphasis is placed on the microstructure of the body, in particular, on the changes that occur during aging. The analysis of the cellular composition of red and white pulp was carried out with the help of the survey methods. It is established that old cats lack a clear division of the spleen follicles into zones. The white pulp follicles of old cats are few and do not have a distinct division between the mantle and marginal zone; they are represented by lymphocytes of B- and T-populations, macrophages (in bright centres and marginal zone) and plasmocytes (mainly in bright centres). Much attention is paid to the morphometric analysis of trabecula and capsules. It is noted that the thickness of the capsule has less variable values, compared with the thickness of the trabecula. In addition, the results of the measurements showed an increase in the thickness of the capsule and trabecula in comparison with mature and young individuals. Morphometric data are provided in summary tables.

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