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VETERINARY SCIENCE

ISSN 2227-6017 (ONLINE), ISSN 2303-9868 (PRINT), DOI: 10.18454/IRJ.2227-6017
ЭЛ № ФС 77 - 80772, 16+

APPLICATION PCR FOR GENOTYPING OF BACTERIA PASTEURELLA MULTOCIDA ISOLATED FROM CATTLE

Posted in 2013, Issue № 9(16) September 2013, VETERINARY SCIENCE | 0 comments

With the help of the developed PCR incidence of serogroups A and D Pasteurella multocida bacteria from the lungs of calves suffering from respiratory diseases was studied. The results can be used in the planning of control measures in the dairy farms, as well as for investigation in molecular epizootology of disease caused by bacteria of the Pasteurellaceae family.

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IMMUNOMORPHOLOGICAL BLOOD PARAMETERS OF RED FOX PUPS (VULPES VULPES) WITH THE INTRODUCTION OF SUBALIN

Posted in 2021, Issue №9 (111) September 2021, VETERINARY SCIENCE | 0 comments

The article presents the results of a study of the effect of the probiotic subalin on the immunomorphological blood parameters of red fox pups at two and eight months of age. The effect of the probiotic was evaluated according to the main hematological, biochemical and immunological blood parameters. As a result of the research, the study demonstrates that the use of subalin for 5 days in all experimental groups of animals has an effect on the immunomorphological blood parameters: in pups at the age of 8 months, the number of red blood cells increases by 9.8%, the level of hemoglobin by 7.2%, and the level of white blood cells decreases by 20-25%, increases the amount of total protein, β -, γ – globulins, bactericidal and phagocytic activity of blood serum.

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THE SPREAD OF ASCARIASIS AND ESOPHAGOSTOMOSIS OF PIGS IN THE CONDITIONS OF FARMING

Posted in 2021, Issue №9 (111) September 2021, VETERINARY SCIENCE | 0 comments

As gathered from relevant literature, ascariasis and esophagostomiasis are severe parasitic diseases caused by helminths belonging to the Nematoda type. Diseases are widespread all over the world and pose a great danger to animals, adversely affecting the productivity of pigs, reducing daily weight gain and increasing feed consumption, which adversely affects the economic condition of farms. Despite the fact that there is a large amount of information on ascariasis and esophagostomosis in pigs presented in the analyzed literature, the issues of the associated course of these invasions remain relevant. In this regard, the authors attempt to study some epizootological aspects of these diseases in pigs in the conditions of outdoor farming in Rostov Oblast. To achieve this goal, the authors set the following tasks: to study the species composition of pig helminths on pig farms and to determine the contamination of the external environment with Ascaris suum and Oesophagostomum dentatum eggs.

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STUDYING THE EFFECT OF ANESTHESIA ON THE MORPHOLOGY OF RED BLOOD CELLS IN DOGS

Posted in 2021, Issue №9 (111) September 2021, VETERINARY SCIENCE | 0 comments

Red blood cells are blood corpuscles, the main function of which is to transfer oxygen to tissues and organs and carbon dioxide in the opposite direction. Since this process is provided by the presence of hemoglobin and a special form of cells, the control of their morphology is very important to ensure normal gas exchange for the body. The aim of the research was to study changes in the shape and size of red blood cells in dogs under anesthesia. Having selected dogs who were brought for planned castration/sterilization at a veterinary clinic, the authors took their blood for research before operation and in a state of anesthesia. The results demonstrate changes in the morphology of red blood cells, which point to the effect of anesthesia on red blood cells.

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THECOURSE AND CLINICAL PICTURE, AGE AND SEASONAL DYNAMICS OF INFECTIOUS KERATOCONJUNCTIVITIS IN CATTLE

Posted in 2021, Issue №8 (110) August 2021, VETERINARY SCIENCE | 0 comments

In the Bryansk region, the Aberdeen Angus breed has become very widespread, it occupies the leading position in the region in terms of numbers. It is a breed of great opportunities; however, it is still not appreciated. In addition to good quality of meat, it has excellent adaptability to severe climatic and forage conditions, cows have a high reproductive ability [1].

Infectious keratoconjunctivitis (Latin — Keratoconjunctivitis) is a polyetiological, acute and rapidly spreading cattle disease characterized by lacrimation, hyperemia of conjunctival vessels, photophobia, serous-purulent discharge, opacity and ulceration of the cornea, deformation of the eyeball. Having conducted an analysis of the available literary sources, the authors come to the conclusion that the research on this topic is insufficient, hence the decision to study this issue.

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FORMS OF THE PROGRESS, AGE DYNAMICS, ASPECTS OF DIAGNOSIS AND TREATMENT OF TRICHOPHYTIA IN DOGS

Posted in 2021, Issue №8 (110) August 2021, VETERINARY SCIENCE | 0 comments

The article describes many aspects of trichophytia in dogs and demonstrates means of combating this common disease with the calculation of their therapeutic and economic effectiveness.

Despite the intensive development of veterinary medicine of small domestic animals, skin pathology in dogs remains the most common and difficult condition to diagnose. According to many researchers [1], [3], [6], currently, more than 25% of veterinary visits of dog owners are associated with a skin disease. The condition of the skin and its derivatives is in many cases an indicator of the overall health of the dog [7].

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CONTROL AND IMPROVEMENT OF VETERINARY AND SANITARY MEASURES IN THE PRODUCTION OF MILK

Posted in 2021, Issue №8 (110) August 2021, VETERINARY SCIENCE | 0 comments

Due to the high contamination of raw milk with mesophilic aerobic and facultative anaerobic microorganisms, the study conducts experiments to improve the veterinary and sanitary treatment of dairy and milking equipment on one of the dairy farms. The results of the research prove that the use of an alkaline detergent and disinfectant “Biotec Super” in combination with an acidic detergent “Ksilan Super” can significantly reduce the amount of bacterial contamination of milking and dairy equipment and is characterized by high economic efficiency and recoupment due to the production of high sanitary quality milk.

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THE EFFECT OF EIMERIOTIC INVASION ON THE ORGANISM OF RABBITS

Posted in 2021, Issue №8 (110) August 2021, VETERINARY SCIENCE | 0 comments

Coccidiosis remains one of the most serious problems of the digestive organs of productive animals, including rabbits. The aim of this research was to determine the degree of pathogenic effect of eimeria at a specific intensity of invasion on the body of purebred and hybrid rabbits.

The scientific experiment was carried out on Chinchilla rabbits and their hybrids with the California breed at the age of 1.5 months. In total, groups of pairs of analogs of 6 heads were formed. Newborn rabbits of the experimental groups were experimentally infected with Eimeria at a dose of about 100 thousand oocysts per head.

On the 45th day of infection, blood samples were taken for biochemical and clinical analysis, animals of experimental and control groups were slaughtered, and the material for histological examination was selected from the small and large intestines, lymph nodes, liver, and spleen.

The results of the research showed that with the intensity of invasion of 100 thousand oocysts per animal, clinical and biochemical blood samples do not reflect the pathological processes occurring in the wall of the small intestine. The authors also did not observe eosinophilia in the blood characteristic of parasite damage; however, there was a slight increase in the percentage of segmented neutrophils compared to the control group animals: in purebred chinchillas from 35.83 ± 0.87 to 36 ± 0.63, and in hybrids – from 42.83 ± 1.08 to 44.67 ± 1.33, which indicated the presence of inflammatory processes and moderate intoxication in the body of infected animals.

A decrease in the albumin content by 29.1-50.19%, depending on the breed, against the background of an increase in the level of globulins in blood plasma, in the opinion of the authors, is due to both inflammatory reactions in the wall of the small intestine, which is accompanied by a violation of the processes of digestion and absorption as well as possible against the background of tissue damage by the influence of toxic products of the breakdown of mucosal cells on the liver. In the small intestine, damage to enterocytes as well as accumulations of lymphocytes and eosinophils were found in histological sections at the sites of localization of meronts and merozoites.

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MORPHOLOGICAL AND BIOCHEMICAL PARAMETERS OF BLOOD IN DOGS WITH DEMODICOSIS

Posted in 2021, Issue №8 (110) August 2021, VETERINARY SCIENCE | 0 comments

This article describes the main forms of demodicosis in dogs, as well as shows changes in morphological and biochemical blood parameters. The research was carried out in the period from 2019 to 2020 at the Department of Parasitology, Veterinary Medical Examination and Epizootology of the Don State Agrarian University and on the basis of veterinary clinics in the city of Shakhty. In the course of the research, it was found that demodicosis in dogs is most often registered in a generalised form (61.14%). When conducting blood tests, the signs of the inflammatory process are most distinct. They are characterized by an increase in the level of white blood cells and the rate of erythrocyte sedimentation, respectively, from 10.8±2,47*109/l with a squamous form up to 27.8±1,59*109/l with a generalised form and 3±3.5 mm/h to 25±3.5 mm/h. At the same time, there is a decrease in the level of red blood indicators as the disease worsens, including a simultaneous increase in the level of ALT and AST to 59.15±1.14 and 70.3±2.45 U/l.

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MODERN DATA OF TRICHINELLOSIS OF THE KHABAROVSK REGION

Posted in 2021, Issue №7 (109) July 2021, VETERINARY SCIENCE | 0 comments

The article analyzes the infection with trichinosis in animals and the incidence of this parasite among residents of the Khabarovsk Territory over the past 5 years. The main source of human infection remains bear meat and wild boar meat. The frequency of infection of bear meat in recent years has varied from 0,5 to 4,45%. Although it previously reached a stable spread of trichinosis due to various environmental and social factors, including poaching. Cases of infection of people (including children) by means of meat of stray dogs are noted. In this regard, it is necessary to strengthen control over spontaneous markets and meat of captured wild animals and inform the population about the possibility of infection with trichinosis.

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AN EVALUATION OF THE LIPOTROPIC EFFECT OF LIVAZEN AGAINST THE BACKGROUND OF TWEEN 80 INDUCED DYSLIPIDEMIA IN RATS

Posted in 2021, Issue №6 (108) June 2021, VETERINARY SCIENCE | 0 comments

Currently, there is an active search for drugs that have a preventive, therapeutic effect with high bioavailability and effective therapy for liver pathology. The study presents the results of using the injectable hepatoprotective drug, livazen, in the modeling of acute hepatitis with the Tween-80 detergent. along with analyzing its effect on the parameters of lipid and carbohydrate metabolism in blood serum in the treatment of experimental hyperlipidemia. The article establishes that livazen has a lipotropic effect and positively affects metabolic processes and improves blood homeostasis.

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BABESIOS DOGS (ANALYSIS OF SCIENTIFIC LITERATURE)

Posted in 2013, Issue October 2013, VETERINARY SCIENCE | 0 comments

The article describes the history of discovery of Babesia canis, morphology pathogen; classification, distribution and specific pathogen carriers babesios dogs outside the Russian Federation and on its territory; the reasons of growth of disease babesios dogs in the city; the form of the clinical course babesios dogs and the possibility of influence of the natural and artificial immunization.

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ONCOLOGIC PATHOLOGIES OF SMALL DOMESTIC ANIMALS

Posted in 2013, Issue October 2013, VETERINARY SCIENCE | 0 comments

In the article the problems of oncological diseases small Pets. Diagnosed growth of neoplastic diseases in dogs and cats for the last ten years. The first place in popularity among tumors, tumors occupy the mammary glands and skin. When conducting pathohistological investigation, we registered the prevalence of malignant forms of neoplasia, over benign, especially cats.

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A STUDY OF THE MEMBRANE-STABILIZING ACTIVITY OF PLANT-BASED DRUGS USING THE PARAMECIUM CAUDATUM TEST SYSTEM

Posted in 2021, Issue №4 (106) April 2021, VETERINARY SCIENCE | 0 comments

The current article presents the results of studying the membrane-stabilizing activity of the plant-based dihydroquercetin and silymarin drugs using biotesting, which assessed the survivability of Paramecium caudatum infusoria. 3% hydrogen peroxide was used as a pharmacological indicator (toxicant), which mainly damages the lipid part of the cell membrane. By way of experiment, the study selected the most effective ratio of dihydroquercetin and silymarin drugs, 1:10, which maximized the time of preserving the mobility and integrity of the cell membrane of the infusoria while the duration of movement of the paramecia in this combination was 4.0 minutes, which is 2.7 times more than the control measurements.

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A STUDY OF BIOCHEMICAL AND MORPHOLOGICAL PARAMETERS OF THE BLOOD OF COWS WITH MASTITIS IN DIFFERENT PERIODS OF LACTATION

Posted in 2021, Issue №3 (105) March 2021, VETERINARY SCIENCE | 0 comments

The article explores the biochemical and morphological parameters of the blood of high-producing cows with subclinical and overt mastitis at different periods of lactation. The research was conducted on 51 Holstein-Simmental cows of the “Druzhba” stud farm. The animals were divided into groups according to the terms of lactation(1st, 3rd-5th, 7th-8th month) and their health condition (clinically healthy, with subclinical mastitis and overt mastitis). The blood was collected for morphological and biochemical analysis from all groups. In the course of the study, it was found that the most significant was the fluctuation in the number of white blood cells in the blood. With the development of subclinical mastitis, the number of white blood cells decreases by 9.6-11.4%, while in the case of overt mastitis, it increases by 1.3 – 9.6% compared to the indicators in groups of healthy animals. The study observes an increased level of nitrogen metabolism, disturbed calcium metabolism, and a decrease in carotene content. The level of immunoglobulins in the blood of sick animals decreases, their concentration is 10.0 to 14.9% less than in clinically healthy animals. The research identifies the absence of a significant influence of the lactation period on the hematological parameters under study, which indicates a high similarity of the course of mastitis in cows at different stages of lactation.

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DYNAMICS OF THE MORPHOLOGICAL PARAMETERS OF BLOOD IN DOGS WITH CANINE HOOKWORM DERMATITIS (UNCINARIASIS)

Posted in 2020, Issue № 12(102) December 2020, VETERINARY SCIENCE | 0 comments

The causative agent of uncinariasis, Uncinaria stenocephala, belongs to one of the most common parasites in the urban dog population and is very widespread.

Canine uncinariasis is a helminthic disease related to nematodes. The transmission of the pathogen can be both foodborne and percutaneous. It is known that during the migration of Uncinaria larvae through the skin, they injure a large number of vessels, tissues and organs of infected animals. In helminthiases, including uncinariasis, all systems of the animal body are involved in the pathological process, including the changes in their haematological status.

The study of haematological parameters is important for the diagnosis of helminthiasis, monitoring the course of the pathological process in the body and establishing the prognosis of the disease. This is the reason for the decision to examine the dynamics of morphological parameters of the blood of dogs infected with Uncinariae before and after deworming.

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EFFICACY OF PARENTERAL NUTRITION IN DOGS WITH CANINE PARVOVIRUS

Posted in 2020, Issue № 12(102) December 2020, VETERINARY SCIENCE | 0 comments

The aim of the research is to study the therapeutic efficacy of parenteral nutrition in dogs with severe conditions of viral infections using canine parvovirus as an example. The research was carried out in the period from 2017 to 2019 at the Department of Non-Infectious Diseases of Farm Animals of the Tyumen/State/Agrarian/University and in the veterinary clinics of Tyumen. To compare the efficacy of parenteral nutrition schemes, the dogs were administered a conventional treatment along with the parenteral nutrition Duphalyte and the combined immunomodulatory drug “Gamavit”. The recovery percentage of the experimental group that was administered Duphalyte equaled 83.3%, in the case of Duphalyte and Gamavit, the recovery percentage equaled 100%, the recovery time amounted to 6.5 days and 4.8 days, respectively.

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A STUDY OF ACUTE AND CHRONIC TOXICITY OF THE BIOPEPTIDE PLUS FEED ADDITIVE ON LABORATORY ANIMALS

Posted in 2020, Issue № 12(102) December 2020, VETERINARY SCIENCE | 0 comments

This article presents the results of a study of the toxicity parameters of biopeptide plus, a complex feed additive. The aim of the research is to identify the negative consequences of intragastric administration of different doses of feed additives in determining acute and chronic toxicity in laboratory animals. The results of the study demonstrate that a single and long-term use of the feed additive in white rats and chickens at a dose of 6000 mg/kg of body weight does not have a negative effect on animal behavior and does not cause any signs of toxicosis. The study shows no critical dose that causes hematological and pathomorphological changes in the organs and tissues of the experimental animals.

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SEASONAL AND AGE DYNAMICS OF CANINE UNCINARIASIS

Posted in 2020, Issue № 11(101) November 2020, VETERINARY SCIENCE | 0 comments

Helminthiasis of domestic carnivores is currently one of the most studied and problematic issues in both veterinary medicine and medicine at large, as many helminths that parasitize domestic carnivores are pathogens of human diseases and agricultural productive animals. [5], [6], [9]. In Russia, toxocariasis, dipylidiasis, toxascaris, and uncinariasis are dominant among canine helminthiases in various rural and urban infrastructures. They pose a serious danger not only to a specific host but also to humans [3], [2], [4]. The analyzed literary sources show that there are virtually no studies of helminths and helminthiasis in carnivores in the Rostov region. In this regard, the decision was made to study the helminth fauna as well as the age and seasonal dynamics of uncinariasis in stray dogs entering the animal control facility in Rostov-on-Don.

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THE “BIOCENOSIS” PROGRAM FOR EARLY DIAGNOSIS OF INFECTIOUS DISEASES OF REPTILES

Posted in 2020, Issue № 11(101) November 2020, VETERINARY SCIENCE | 0 comments

The article contains a study of the microbiocenoses of various biotopes of reptiles belonging to the following species: corn snake (Pantherophis guttatus), Russian rat snake (Elaphe schrenckii), desert sand boa (Eryx miliaris). In the biotopes of these reptile species, various microorganisms were identified, such as coccoid forms of bacteria, mold fungi, bacteria of the Escherichia coli group, Pseudomonas aeruginosa. The study of the dynamics of microbiocenoses in reptiles makes it possible to model changes in microbiocenotic communities with various indicators of microclimatic parameters. By modeling changes in microbiocenoses, the authors developed a program for the early diagnosis of infectious diseases consisting of a number of algorithms based on the collected database.

For the program to work correctly, it is necessary to study microbiocenoses and the sensitivity of microflora to antibacterial drugs. The cost of the study of one animal is 2,088 rubles. The cost of materials required for the analysis of one animal is 793.25 rubles. The amount required for the normal operation of the “Biocenosis” program to track changes in microbiocenotic communities in animals is 14,619. rubles annually. While the total cost of treatment of a reptile from a chronic infectious disease amounts to 7,170 rubles per reptile in the course of 3 months, the annual amount will be 28,680 rubles. This program helps prevent the following: infection of the host with particularly dangerous forms of pathogenic bacteria; death of the animal; the ability to save up to 14,000 rubles a year for the treatment of a single animals’s chronic infectious diseases.

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