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BIOLOGY

ISSN 2227-6017 (ONLINE), ISSN 2303-9868 (PRINT), DOI: 10.18454/IRJ.2227-6017
ПИ № ФС 77 - 51217, 16+

DYNAMICS OF CHANGE OF SEROTONIN CONTENT IN THE CNS OF RABBITS AT DIFFERENT STAGES OF FOOD DEPRIVATION AND ITS CANCELLATION

Posted in 2019, BIOLOGY, Issue № 6(84) June 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

The purpose of this work was to study the serotonin content in the mitochondria of the brain structures of 3-month-old rabbits at different stages of food deprivation and against the background of the restoration of the feeding schedule. The experimental group was divided into subgroups with regard to the duration of the period (1, 3 and 5 days) of food deprivation and against the background of a 7-day restoration of the food schedule. Forced food deprivation causes a decrease in serotonin levels in the mitochondrial fractions of the brain in 3-month-old rabbits.

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DYNAMICS OF GABA METABOLISM IN RAT’S BRAIN STRUCTURES WITH EXPOSURE OF TOLUENE

Posted in 2019, BIOLOGY, Issue № 6(84) June 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

The authors consider the dynamics of GABA metabolism in tissues of various CNS structures in intact 6-month-old rats after exposure of toluene (5 days, intraperitoneally, 500 mg/kg). The results of this research have shown that when exposed to toluene, there is an increase in the content of GABA, a decrease in the content of free Glu and Asp, an increase in the activity of the GDC enzyme and a decrease in the activity of the GABA-T enzyme in the tissues of brain structures of 6-month-old rats. It can be assumed that toluene has an effect on the protein structures of GABA metabolism enzymes or interacts with their coenzyme, pyridoxal-5-phosphate.

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STUDY OF DERMATOGLYPHYCHIC AND SOMATOMETRIC INDICATORS OF WATER POLO PLAYERS OF THE TEENAGE PERIOD OF VARIOUS PLAYING ROLES

Posted in 2019, BIOLOGY, Issue № 5(83) May 2019 | 0 comments

The connection of the somatotypological and dermatoglyphic characteristics of athletes with their playing role was determined in the study. Forwards often have a “whirl” finger pattern, while the “loop” pattern dominates for defenders. Forwards exceed water polo players of other game roles in the overall body size, they have a little bit larger girths and larger absolute contents of muscle and fat components. Defenders are characterized by a greater length of a skeleton; their characteristic feature was the large lengths of the upper and lower extremities and their links. Also, in the study, it was revealed that the water polo players, who differed in game function, had different body types. Thus defenders have ecto-mesomorphic (60%) and meso-ectomorphic (40%) types of constitution, forwards have a greater variety identified somatotypes: endo-ectomorphic (40%), meso-endomorphic (30%), endo-mesomorphic (20%), mesomorphic (10%).

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CHANGING DYNAMICS OF BIOGENIC AMINES CONTENT IN HYPOTHALAMUS OF RABBITS WITH DIFFERENT TIMES OF FOOD AND DRINKING DEPRIVATION AND ITS CANCELLATION

Posted in 2019, BIOLOGY, Issue № 5(83) May 2019 | 0 comments

The goal of this work is to study the content of biogenic monoamines (dopamine, norepinephrine, and serotonin) in the hypothalamus of 3-month-old rabbits exposed to food and drinking deprivation. The results of the research showed that depending on the timing of food and drinking deprivation, the content of dopamine, norepinephrine, and serotonin undergoes significant changes.

Drinking deprivation, as compared to nutritional, causes more significant changes in the content of biogenic monoamines in the hypothalamus.

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THYMOANALEPTIC EFFECT OF SAFFRON EXTRACT IN ANIMALS WITH TYPOLOGICAL STATUS

Posted in 2019, BIOLOGY, Issue № 5(83) May 2019 | 0 comments

This paper is devoted to the study of the correctional effect of the saffron extract on behavior parameters in the experimental model of depression in rats with a typological status. The results of the conducted studies show that the effect of the saffron extract is manifested by an increase in locomotor and orienting activity; and a decrease in anxiety. During the experiment, the depressive indicators decrease almost to the control level. The obtained result suggests that the depressive state is corrected by chronic administration of saffron extract and the restoration of the functional status of previously selected rats prone to depression is accelerated.

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GROWTH RHYTMICITY OF ANNUAL TWIGS OF ALMOND-LEAVED WILLOW (SALIX TRIANDRA L.) AS PESTICID LOAD OPTIMIZATION FACTOR IN INTENSIVE TYPE PLANTING

Posted in 2019, BIOLOGY, Issue № 5(83) May 2019 | 0 comments

The authors present general characteristics of almond-leaved willow (Salix triandra L.): taxonomy, cytogenetics, practical importance as a source of twig for weaving. The authors analyzed the possibility of genetic protection of almond-leaved willow twigs from pathogens and pests in the paper. The necessity of the use of pesticides with a prolonged effect on the twig plantations of the intensive type has been substantiated. In order to reduce the pesticide load, they proposed to plan agroforestry measures which take into account the rhythms of development of annual twigs. In the model inbred population of almond-leaved willow seedlings of the third generation, the patterns of seasonal cyclicity of changes in the length of internodes were revealed with the use of amplitude-phase analysis. It is proved that seasonal trends in the dynamics of the length of internodes depend on the individual characteristics of seedlings and are due to the interaction of the lower unsynchronized harmonics with the oscillation period of 48…144 (288) days. The cyclical nature of the seasonal dynamics of the length of the internode, which does not depend on the individual characteristics of seedlings, in most cases is determined primarily by synchronized harmonics with a period of oscillations of 36 days and modulating harmonics with a period of oscillations of 24 days. Higher harmonics with a period of oscillations of 10…21 days have a slight modulating effect on the seasonal dynamics of the length of the internode. To optimize the pesticide load, they proposed to carry out triple treatment of plantations with a frequency of about 36 days.

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MULTIDIMENSIONAL ANALYSIS OF WATER QUALITY PARAMETERS OF ISSYK-KUL LAKE WATER

Posted in 2019, BIOLOGY, Issue № 5(83) May 2019 | 0 comments

This article presents the results of water quality multidimensional analysis of the water form Issyk-Kul Lake, conducted in 2014-2015 with the use of MINITAB statistical method (version 16). With the development of technology, it became possible to identify the productive zones of Issyk-Kul Lake by sites. It was found that Issyk-Kul Lake belongs to the group of oligo-alkaline reservoirs, where the average pH is 8-9. The total mineralization of water form this lake ranges from 3.4 to 4.3 mg/l, according to V.I. Vernadsky classification, it proves that Issyk-Kul Lake can be considered a saltish water basin. According to the classification of P.F. Domrachev, Issyk-Kul Lake is considered to be a warm water body, where the average integral water temperature in Issyk-Kul Lake is from 17.2 to 22.1°C in summer [1]. The air temperature above 15°C in Issyk-Kul region lasts 106-120 days a year [2].

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GENERATION AND CHARACTERIZATION OF THE VP1 RECOMBINANT PROTEIN OF THE CHICKEN ANEMIA VIRUS

Posted in 2019, BIOLOGY, Issue № 5(83) May 2019 | 0 comments

The optimized sequence of the VP1 gene fragment of the infectious chicken anemia virus was cloned into the expression vectors pET15b and pGEX-3T in-frame fused with polyhistidine and glutathione-S-transferase tags, respectively. E.coli strains Rosetta (DE3) producing recombinant 6HIS-ΔVP1 and GST-ΔVP1 proteins were obtained. The 6HIS-ΔVP1recombinant protein purification was performed using Immobilized Metal Affinity Chromatography; the refolding conditions, ensuring the specific interaction of the protein with the control polyclonal antibodies, were fulfilled.

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BIODIVERSITY OF TAIGA TREES WITH DARK NEEDLES IN ATSA RIVER BASIN (EASTERN TRANSBAIKAL)

Posted in 2019, BIOLOGY, Issue №4(82) April 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

The results of the study of trees with dark needles in Atsa River basin forming part of the catchment area of the Lake Baikal basin are generalized in the article. At present, forest ecosystems of the Lake Baikal basin undergo strong changes under the influence of anthropogenic and natural factors. In this connection, the tasks were clearly set: To describe the biocoenosis of trees with dark needles of the Atsa River basin, to evaluate their species diversity, to make preliminary forecasts of changes in biodiversity. For the purpose of finding the least anthropogenically (including pyrogenically) impacted tees with dark needles, well-preserved taiga massifs of the Atsa River basin of 50° north latitude and of 109° eastern longitude at an altitude of 930-1600 m above sea level were considered. As a result of the studies, about 100 species of higher vascular plants were detected in the flora of trees with dark needles. It suggests that trees with dark needles are not inclined to form plantations with a large impurity of plants. The smallest species richness of vascular plants is characterized by spruce biocoenosis and pine forests, fennel, rhododendron and blueberries. From the point of view of the rare species growth included in the Red Book of the Transbaikal region (Eastern Transbaikal), trees with dark needles contain one species – Sorbus sibirica.

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BIOCENOSES OF SOME BLACK SEA RIVERS

Posted in 2019, BIOLOGY, Issue №4(82) April 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

A comprehensive description and analysis of the qualitative and quantitative characteristics of the zoonoses of seven small rivers of the Black Sea coast of the Caucasus were carried out for the first time based on the results of studying the main groups of hydrobionts (phytoplankton, zooplankton, zoobenthos, and fish). The study of ichthyofauna is of both scientific faunistic interest and has practical significance in the study and preservation of biological diversity under the conditions of powerful anthropogenic impact.

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DEGREE OF INCIDENCE OF HUMAN PAPILLOMA VIRUS GENOTYPES OF ONCOGENOUS RISK BY THE RESULTS OF RETROSPECTIVE AND PROSPECTIVE ANALYSIS IN THE CITY OF BAKU

Posted in 2019, BIOLOGY, Issue №4(82) April 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

The main goal of this study is to identify the degree of incidence of oncogenic types of human papillomavirus (HPV) in patients with cervical cancer (CC) anda healthy population of Baku, as well as to determine the relationship between the frequency of detection of these genotypes with the clinical form of HPV. Retrospective (210 patients) and prospective (206 patients) analysis were performed. Women in the acute period of associated diseases and pregnant were excluded from this study. The samples of epithelial scrapings from the cervical canal and cervix were taken from all women were the material for the study. In order to identify the relationship between the frequency of oncogenic HPV types in patients with cervical cancer and the clinical form of the course of HPV 210 patients were divided into two groups forretrospective analysis. The first group included patients with manifest HPV (20.0 ± 2.5%), the second group included patients with subclinical and latent HPV (80.0 ± 2.6%). It was found that in patients with CC, the genotype of HPV-16 is significantly more common, both as a separate genotype and in combination with other genotypes of medium and high oncogenic risk. A prospective study conducted among a healthy population of women revealed that the incidence of HPV in a healthy population was set at 8.7%. There is also a high risk of genital HPV infection (type 16 and type 18).

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INFLUENCE OF OIL POLLUTION ON ENZYME ACTIVITY AND CELLULOSE-DECOMPOSING MICROORGANISMS IN GRAY-DRILLED SOIL

Posted in 2019, BIOLOGY, Issue №3(81) March 2019, Issue №3(81) March 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

This scientific article is devoted to the study of the impact of fresh oil pollution (Surakhani deposits) on the dynamics of microbiological processes in the soil, including cellulose-decomposing microorganisms. In order to understand how this happens and what factors affect it, a field monitoring of the contaminated site was conducted, it showed that the depth of penetration of crude oil was up to 12-13 cm. The results of chemical analysis of soil samples showed that the oil content in the soil was 2,1 g/100 g of soil. The number of microorganisms per MPA (meat-and-peptone agar) capable of decomposing hydrocarbons (using n-hexadecane), the number of cellulolytic microorganisms in Getchinson’s medium, the degree of phytotoxicity (germination of watercress seeds), and the degree of soil pollution by oil were also determined. The authors investigated the consumption of oil and petroleum products by CDM culture. It was revealed that after oil pollution there is a tendency to reduce the total number of microorganisms growing on MPA (heterotrophic groups of microorganisms) and cellulose-decomposing microorganisms against the background of the increase in the number of hydrocarbons of oxidizing microorganisms. The decomposition of cellulose was considered by various cultures of CDM, and it was revealed that the culture isolated from virgin types of ecotopes (the territory of the Dzheyranbatan reservoir) had the highest activity for the decomposition of cellulose. In another series of model experiments, the effect of a biopreparation based on associative polyfunctional bacteria No. 2, No. 7, No. 23 on the biogenicity of oil-contaminated soil and the intensity of oil decomposition was studied. The task of the experiment was to test cellulolytic bacterial strains, which confirmed the effectiveness of inoculation of seeds of higher plants, and that creates the scientific basis for the creation of bacterial fertilizers using cellulolytic bacteria. These biological products can be very effective in the development of technologies for phytoremediation of oil-contaminated gray-brown soils.

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FEATURES OF SHORT-TERM MODIFICATION OF BACKGROUND RHYTHM DEPENDING ON THE MOMENT OF ECTOGENOUS SENSORY AFFERENTATION ENTRY AT DIFFERENT PHASES OF THE ENDOGENOUS ALPHA RHYTHM

Posted in 2019, BIOLOGY, Issue №1(79) January 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

It was proven with the help of 20 practically healthy subjects of both sexes that photo stimulation synchronized with the ascending phases of alpha waves leads to a “breakdown” of the alpha rhythm at the 150th ms of the post-stimulus period. The presentation of the stimulus in the descending phase of the alpha wave reorganizes the endogenous alpha rhythmic activity in the 200th ms of the post-stimulus period. The observed changes are associated with the state of potential-dependent ion channels that form slow pacemaker potentials of cortical neurons.

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DISTRIBUTION OF CROP WILD RELATIVES PLANTS INTO ECONOMICALLY IMPORTANT GROUPS IN THE REPUBLIC OF KARAKALPAKSTAN

Posted in 2019, BIOLOGY, Issue №2(80) February 2019 | 0 comments

Motivation: Species of Crop wild relatives plants are not the same by the degree of usage by human and economic importance. The major part is represented by broadly spread forages, populations of which are introduced equally stabilized conditions. However a number of species belong to various categories of rarity and vulnerability i.e need protection. A definite part of these species in a high priority in selection process, but which also need immediate protective measures as great part of ingredient source of podder resources.

Results: The analysis of composition of higher vascular plants are found within the limits of the Republic of Karakalpakstan the compendium of crop wild relatives plants consist of 24-families, 85-sorts and 128-species, and educed a presence at least 6 different economic-valuable groups of these species. From them: 102-forage, 43-food, 31-melliferous, 54-medicinal and 33-technical species of plants.

Availability: The results of researches will enable more deeply to analyze structures of crop wild relatives cultural plants of the Republic of Karakalpakstan. The data can be used at drawing up of qualifiers, at reading lectures and realizations of practical classes in high schools, colleges, Lyceums.

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FEATURES OF COENOTIC POPULATIONS STRUCTURE OF CYPRIPEDIUM CALCEOLUS L. (ORCHIDACEAE) IN SAMARA REGION

Posted in 2019, BIOLOGY, Issue №1(79) January 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

Information about the structure of coenotic populations of rare plants is important in the assessment of the current state of the species in the regions and throughout the area, as well as in the development of measures for the protection of natural complexes. The ontogenetic structure of coenopopulations of a rare plant, Cypripedium calceolus L. (Orchidaceae), in the Samara region is studied. It is necessary to mention the features of ontogenesis and anthropogenic factors among the main factors limiting the increase in the type of factors. Base ontogenetic spectrum is full-term centered with a predominance of mature generative individuals (44.8%). The spectra of the specific coenopopulations under study are often different from the base one and are right-handed. About 60% of coenopopulations are aging, 40% are mature (taking into account the age and efficiency criteria of populations).

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FORMATION OF CAUDEX AND DEVELOPMENT OF PARTITIONING OF UNDERGROUND ORGANS OF SOME STEPPE SPECIES OF PLANTS IN SAMARA REGION

Posted in 2019, BIOLOGY, Issue №1(79) January 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

The steep slopes of beams, ravines, syrts, ridges, river valleys of the Samara region often consist of sediments of the red clay of the Permian system and the Triassic. The characteristics of the soils determine the main features in the development of the underground organs of plants. Many steppe perennial plants of the Samara region form caudex, which is subject to partitioning. However, the partitioning was also noted in the representatives that do not form caudex. We recorded the partitioning of the underground organs (root, rhizome) in the generative (and subsequent) period of ontogenesis in 24 model plant species. In this case, only 19 representatives have caudex. This confirms that the term “partitioning” is very widely interpreted in modern plant morphology.

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NUTRITION OF BLACK BAIKAL GRAYLING (THYMALLUS BAIKALENSIS) IN LAKE SHEBETY (TRANSBAIKAL TERRITORY)

Posted in 2019, BIOLOGY, Issue №1(79) January 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

The paper presents materials on nutrition of the black Baikal grayling obtained in the summer period of 2016. 12 components were noted in the stomachs of grayling. The basis of the diet, however, consisted of chironomid larvae. The change in the ratio of the main components of food depending on the habitat was revealed. Chironomid larvae dominated in the ration of the grayling in the deep-sea part, and the representatives of aerial insects and zooplankton organisms dominated at a depth of 5 m. Fish were characterized by a relatively high degree of filling, which indicates the availability of this type of food resources.

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MAIN FEATURES OF MORPHOLOGICAL AND FUNCTIONAL PARAMETERS OF ERYTHROCYTES IN HEALTHY GIRLS WITH DIFFERENT I/D GENOTYPES OF GENE POLYMORPHISM OF ANGIOTENSIN-CONVERTING ENZYME

Posted in 2019, BIOLOGY, Issue №1(79) January 2019, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

The main features of the quantitative morphological and functional characteristics of erythrocytes, as well as their relationships among the owners of different genotypes of the insertion-deletion J/D polymorphism gene of the angiotensin-converting enzyme (ACE), were studied. Genotypic differences in the size of erythrocytes, their fullness with hemoglobin, and osmotic resistance are established. In the presence of the J allele in the genotype, a connection was demonstrated between the number of erythrocytes, hematocrit with the JJ genotype, hemoglobin content and the stability of erythrocyte membranes.

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СOMMUNICATION OF PARAMETERS OF HATCHING EGGS WITH HATCHABILITY AND SEX DETERMINATION OF BIRDS

Posted in 2018, BIOLOGY, Issue №12(78) December 2018 | 0 comments

The purpose of this research was to study of effect of periods of storage of eggs before incubation, their shape, mass and density on hatchability, and sex ratio. The increaseof hatchability and early detection of sex determination of young birds create opportunitiesfor differentiation of their keeping and feeding that will help to reduce the cost of poultry products and increase of industry profitability. Interconnection of hatchability and by the studied parameter. Sex of pauls has been determined by Japanese method.According to the results of study of incubation of turkeys eggs have been found that sue to the elongation of shelf life from 7 to 9 days, there was a decrease of hatching of young turkeys from 72.2% to 68.2%; geese eggs with a shelf life from 7-14 days – from 64.6% to 55.2% with ratio change in hatching of females/males from 1.00/.02 to 1.00/1.26 up to 1.00/1.03 and 1.00/1.21 respectively. Hatchability of turkeys’ eggs with weight 80-90 g was 75.4%, geese eggs with weight 140-150 g – 66.7%. Upon gaining in weight of incubated eggs of turkeys from 80 to 90 g and ratio of female/male has been increased from 1.00/0.96 to 1.00/1.11 and geese from 140 to 150 g or more to 1.00/1.06 to 1.00/1.13. When the index of shape of eggs is reduced, the hatching of young birds decreased and density of males increased. The decrease in density also reduced the hatching of young birds, but does not reflect on sex ratio.

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INFLUENCE OF ADVANCED GLYCATION END PRODUCTS ON CELLULAR PROCESSES

Posted in 2018, BIOLOGY, Issue №12(78) December 2018, БИОЛОГИЧЕСКИЕ НАУКИ | 0 comments

Advanced Glycation End products or AGEs are formed as the result of non-enzymatic interaction of proteins with carbohydrates and belong to the group of modified amino acid residues that can slowly accumulate in the body during the normal process of aging. In addition to endogenous AGEs, these toxic compounds appear in the human body due to the consumption of food that has been heat treated or subjected to long-term storage, due to active and passive smoking. AGEs can cause the onset and progression of complications of diabetes, and become the basis of the pathophysiology of many other diseases including cardiovascular and neurodegenerative ones. Both exogenous and endogenous AGEs play a significant role in the development of the inflammatory response in the human body. The proposed review examines the structure and functions of the main AGEs receptors and the signaling pathways they activate, as well as some of the biological effects of this activation.

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