СТРЕСС-ИНДУЦИРОВАННЫЕ ИЗМЕНЕНИЯ В ПРОТЕОМНЫХ ПРОФИЛЯХ МИНДАЛИНЫ И КОРЫ ГОЛОВНОГО МОЗГА КРЫС С РАЗЛИЧНЫМИ ХАРАКТЕРИСТИКАМИ ПОВЕДЕНИЯ В УСЛОВИЯХ МЕТАБОЛИЧЕСКОГО СТРЕССА И ПРИ ВВЕДЕНИИ В РАЦИОН КОЭНЗИМА Q10
Кирбаева Н.В. 1, Шаранова Н.Э. 2, Васильев А.В. 3
1 ORCID: 0000-0001-6623-6911, аспирант, Федеральное государственное бюджетное научное учреждение «Научно-исследовательский институт питания» (ФГБНУ «НИИ питания»), 2 ORCID: 0000-0003-2007-5686, Кандидат биологических наук, ФГБНУ «НИИ питания», 3 ORCID: 0000-0002-7808-4799, Доктор биологических наук, ФГБНУ «НИИ питания»
СТРЕСС-ИНДУЦИРОВАННЫЕ ИЗМЕНЕНИЯ В ПРОТЕОМНЫХ ПРОФИЛЯХ МИНДАЛИНЫ И КОРЫ ГОЛОВНОГО МОЗГА КРЫС С РАЗЛИЧНЫМИ ХАРАКТЕРИСТИКАМИ ПОВЕДЕНИЯ В УСЛОВИЯХ МЕТАБОЛИЧЕСКОГО СТРЕССА И ПРИ ВВЕДЕНИИ В РАЦИОН КОЭНЗИМА Q10
Аннотация
В статье рассмотрены изменения в протеомных профилях отделов головного мозга крыс (миндалина, кора головного мозга) в условиях метаболического стресса на модели голодания. Показано повышение и понижение экспрессии кальцинейрина B гомологичного белка 1, пероксиредоксина 2, глутатион-S-трансферазы омега 1, OTUB1 и ряда других белков в зависимости от поведенческого типа животного и стадии эксперимента. Установлено влияние дополнительного приема в составе диеты коэнзима Q10 на организм стрессированных животных. Полученные результаты демонстрируют влияние индивидуальных поведенческих характеристик животных на специфические пути реакции организма на стресс и определение адаптивного потенциала организма.
Ключевые слова: крысы, мозг, поведение, протеомика, метаболический стресс.
Kirbaeva N.V. 1, Sharanova N.E. 2, Vasil’ev A.V. 3
1 ORCID: 0000-0001-6623-6911, Postgraduate student, Federal State Budgetary Institution «Institute of Nutrition», 2 ORCID: 0000-0003-2007-5686, PhD in Biology, Institute of Nutrition, 3 ORCID: 0000-0002-7808-4799, PhD in Biology, Institute of Nutrition
STRESS-INDUCED CHANGES AND EFFECT OF COENZYME Q10 SUPPLEMENTATION IN AMYGDALE AND CORTEX PROTEOMIC PROFILES OF RATS WITH DIFFERENT BEHAVIORAL CHARACTERISTICS UNDER THE CONDITIONS OF ACUTE METABOLIC STRESS
Abstract
This study aims to investigate the changes in proteomic profiles of brain areas (amygdale, cortex) in rats under the conditions of acute metabolic stress. The up/down-regulation calcineurin B homologous protein 1, peroxiredoxin 2, glutathione S-transferase omega 1, OTUB1 and some other proteins was shown depending on the behavioural type of rats and the stage of experiment. The effect of coenzyme Q10 supplementation on stressed rat’s organism was indicated. The results demonstrated that individual behavioral features affect the specific pathway of organism response to the stress and determine an adaptive potential of the organism.
Keywords: rats, brain, behavior, proteomic, metabolic stress.
The cerebral cortex plays a very important role in the implementation of higher nervous (psychological) activity and the prefrontal cortex plays a primary role in translating stressful emotional information into action. It has been shown that stress produces neurochemical and behavioral changes associated with prefrontal cortex function [4]. Evidence from animal studies has demonstrated that the limbic system is initially involved in emotional responses. The amygdale is the most multifunctional structure of the limbic system and affects the stress-dependent behavior, initiating emotionally motivated responses [3].
The development of many metabolic and immune disorders, obesity and premature aging is closely related to the lack of energy production in the body and damage of cellular energy generators [2]. One of the most important participants of electron transport coupling and oxidative phosphorylation is a coenzyme Q10 (CoQ10). Physical and emotional loads and chronic stress leads to increased consumption of endogenous CoQ10, causing a high probability of its deficit in the organism [1].
To identify the differences of proteomic expression in rat brain caused by acute metabolic stress and under the CoQ10 additional usage we performed comparative proteomic analysis.
Wistar male rats were divided on 2 groups of behaviorally passive and active animals using the classical Open field test. Starvation of rats (water ad libitum) during 5days served as a model of acute metabolic stress. There was a 5day recovery period after the starvation, while animals received a standard diet. Some of the groups consumed CoQ10 at the dosage of 10 mg/kg bw in the diet. Thus, behaviorally active and passive rats were divided into 14 groups with 8 rats in each one. The protein expression profiles of amygdale and cortex were studied by using two-dimensional electrophoresis and MALDI-TOF.
In amygdale of active rats the proteomic analysis showed the down-regulated expression of calcineurin B homologous protein 1, peroxiredoxin 2 at the stage of stress, tropomodulin 2, glutathione S-transferase omega 1 at the stage of stress and recovery periods; up-regulated expression of Tcrb protein in recovery period and GTP-binding protein SAR1a in periods of stress and recovery. In amygdale of passive rats down-regulated expression was shown in case of NADH dehydrogenase [ubiquinone] Fe-S protein 8, peroxiredoxin 2 and glutathione S-transferase omega 1 at the stage of stress, tropomodulin 2 in stress and recovery periods, calcineurin B in the recovery period; up-regulated expression was shown in case of Tcrb protein in stress period, Ras-related protein Rab-14 at the stages of stress and recovery, GTP-binding protein SAR1a at the recovery stage.
In the case of additional CoQ10 supplementation in the diet of active rats the proteomic analysis showed the down-regulated expression of calcineurin B homologous protein 1 in control group and Tcrb protein in recovery period; up-regulated expression of GTP-binding protein SAR1a in control period, calcineurin B homologous protein 1, Tcrb protein and peroxiredoxin 2 at the stage of stress; tropomodulin 2 in recovery period. In amygdale of passive rats it was shown the down-ragulated expression of calcineurin B homologous protein 1 in control period, peroxiredoxin 2 in control and recovery periods, GTP-binding protein SAR1a at the stage of recovery; up-regulated expression of NADH dehydrogenase [ubiquinone] Fe-S protein 8 in stress group, GTP-binding protein SAR1a in control period, calcineurin B homologous protein 1 and Tcrb protein at the stage of recovery, peroxiredoxin 2 and glutathione S-transferase omega 1 in stress period.
In cortex of active rats the proteomic analysis showed the down-regulated expression of NADH dehydrogenase [ubiquinone] flavoprotein 2 in the stress period, OTUB1 at the stage of stress and recovery; up-regulated expression of serine/threonine protein phosphatase 1 (PP1) in the recovery period. In cortex of passive rats down-regulated expression was shown in case of OTUB1 and NADH dehydrogenase [ubiquinone] flavoprotein 2 at the stage of stress and stress and recovery respectively.
In the case of additional CoQ10 supplementation in the diet of active rats the proteomic analysis showed the down-regulated expression of alpha-synuclein and Ras-related protein Rab-1A in recovery period; up-regulated expression of serine/threonine protein phosphatase 1 (PP1) in control group, NADH dehydrogenase [ubiquinone] flavoprotein 2 in the stress period and OTUB1 in recovery period. In cortex of passive rats with additional usage of CoQ10 the proteomic analysis showed the up-regulated expression of OTUB1 in control and stress periods and NADH dehydrogenase [ubiquinone] flavoprotein 2 only in recovery period.
These findings indicate the formation of specific proteomic profile of animals in conditions of acute stress. The additional usage of CoQ10 results in some recovery of the negative effects of the stress on the rat’s organism.
Thus, individual behavioral features affect the specific pathway of organism response to the stress and determine an adaptive potential of the organism.
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