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ISSN 2227-6017 (ONLINE), ISSN 2303-9868 (PRINT), DOI: 10.18454/IRJ.2227-6017
ПИ № ФС 77 - 51217

DOI: 10.18454/IRJ.2016.53.174

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Пальчиков А. Ю. ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ НОВОЙ ВИТАМИНО-ФЕРМЕНТНОЙ ДОБАВКИ В РАЦИОНАХ СЕЛЬСКОХОЗЯЙСТВЕННОЙ ПТИЦЫ / А. Ю. Пальчиков, Л. В. Резниченко, С. В. Воробиевская и др. // Международный научно-исследовательский журнал. — 2016. — № 11 (53) Часть 2. — С. 159—161. — URL: (дата обращения: 23.01.2017. ). doi: 10.18454/IRJ.2016.53.174
Пальчиков А. Ю. ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ НОВОЙ ВИТАМИНО-ФЕРМЕНТНОЙ ДОБАВКИ В РАЦИОНАХ СЕЛЬСКОХОЗЯЙСТВЕННОЙ ПТИЦЫ / А. Ю. Пальчиков, Л. В. Резниченко, С. В. Воробиевская и др. // Международный научно-исследовательский журнал. — 2016. — № 11 (53) Часть 2. — С. 159—161. doi: 10.18454/IRJ.2016.53.174



Пальчиков А.Ю.4 , Резниченко Л.В.1, Воробиевская С.В.2Сыровицкий В.А.3

1ORCID: Доктор ветеринарных наук, профессор, 2ORCID: Кандидат биологических наук, доцент, 3ORCID: Кандидат сельскохозяйственных наук, доцент   ФГБОУ ВО «Белгородский государственный аграрный университет им В.Я. Горина», 4ORCID: Начальник отдела государственного ветеринарного надзора на государственной границе, транспорте и внутреннего ветеринарного надзора Управления Россельхознадзора Белгородской области



В статье рассматривается проблема  обеспечения цыплят-бройлеров новой эффективной витамино-ферментной добавкой. Установлена высокая фармакологическая  эффективность применения стимулара  цыплятам-бройлерам, которая проявлялась увеличением среднесуточных приростов птицы и повышением  конверсии корма. После применения изучаемой кормовой добавки в сыворотке крови цыплят произошло увеличение белка, витамина А  и кальция. В пределах физиологической снизилась активность ферментов перенаминирования. Для увеличения продуктивности сельскохозяйственной птицы можно рекомендовать вводить в рационы цыплят-бройлеров  стимулар из расчёта 1,0 г/кг массы тела на протяжение всего периода выращивания.

Ключевые слова: цыплята-бройлеры, витаминно-ферментная добавка, аспартатаминотрансфераза.

Palchikov A.Y.4, Reznichenko L.V.1, Vorobievskaya S.V.2, Syrovitskiy V.A.3

1PhD in Veterinary Medicine and Science, Professor, 2PhD in Biology, Associate Professor, 3PhD in Agriculture, Associate Professor FSBEI of Higher Education «V. Gorin Belgorod State Agriculture University», 4Chif of branch of State veterinary surveillance on the state border, transport and internal veterinary surveillance of Directorate of the Rosselhoznadzor for the Belgorod Oblast



The problem of providing broiler chickens with new effective vitamin-enzyme premix came into review in this article.  High pharmacological efficiency of stimular usage on broiler chickens was observed and was evidenced in avarege daily increasing of poultry and rising of feed conversion. After the usage of examined feed premix in blood serum of broiler chickens was increased proteic substance, vitamin A and calcium. Alkaline phosphatase activity was decreased within normal physiological limits. For the increasing of poultry productivity stimular is possible to recommend to add into broiler chickens rations assuming 1,0 g/kg bwt during the whole raising period. 

Keywords: broiler chickens, vitamin-enzyme premix, alkaline phosphatase (AP).


Increasing of the poultry production yielding depends significantly not only on fodder base quality, but also on efficient use of   feed availability. It is known, that   full value condition of ration is possible to provide by adding to it bio-effecting agents and enzymes [3, 4].

In poultry breeding as basic concentrated feeds are used barley, oats, rye, maize, non-food wheat and its derivatives. Low food-value of some of the cereals is due to the fact that together with fibre they massively contain other non-starch polysaccharides, to which belong beta-glucans and pentosanes. They can be found in cell walls of cornel albumen and are not removed while processing. Beta-glucans adversely affect to nutrient intake in barley, pentosans – in wheat, rye and triticale [5].

Feed rations breeding with enzyme preparations minimizes young stock mortality, significantly increases fodders take and decreases its unit cost, allows partly replace expensive and deficit fodders of animal origins with cheaper of vegetable ones, and also allows to increase poultry productivity while simultaneous improvement of obtainable production quality [1, 6].

Consequently, necessity of using in poultry rations effective vitamin-enzyme complexes comes to our heads. From there we together with staff members of CJSC “Petrokhim” produce new vitamin-enzyme premix stimular.

The aim of this study was to examine stimular influence over broiler chickens organisms for its practical application as source of vitamins, enzymes and calcium.  

Stimular – feed vitamin-enzyme premix, contained fermentolysate of lien (70% mass); pepsin (2,6% mass); fodder lime (26,85% mass) and vitamin premix (3%) at 1g of stimular: vitamin A -500ME, vitamin D3 – 44ME, vitamin E – 0,7 mg, B vitamins (B1 – 0,17 mg, B2 – 0,17 mg, B6 – 0,18 mg, B12 – 0,36μ), vitamin PP – 2mg, folic acid – 0,06mg, pantothenic – 0,8mg, biotin – 0,022mg, vitamin C – 9mg.

Material and methods

Pharmacological effectiveness of stimular was identified by broiler chickens from «Hubbard».

About stimular influential character to animals organisms we went by clinical score, changing of protein, carbohydrate, mineral metabolism, general non-specific organism resistance, growth intensiveness and         productivity of livestock [2]. Trials were taken place in Belgorod region in on-the-farm conditions.

Group forming was carried out according to analogues principle. Biochemical incites was identified according to standard methods. By doing that Hematology Analyzer “Hitachi” was used.   Lysozyme activity in blood serum was found out using        turbidimetric method (V. Dorophejchuk, 1968), phagocytic activity by phagocytizing neutrophils counting from 100 organules,   blood serum bactericidal activity – according to I. Karput [2].

Obtained in all experiments digital information was subjected to statistical analysis on the personal computer according to standard methods of     variation statistic with evaluation of Student’s argument (td). The difference between compared quantities was considered to be authentic, when р0,05 (G. Lackin, 1973).

Results and discussion

For conducting of the trial of growth-stimulating effect of stimular there were formed 2 groups with broiler chickens 14-days old with 50 heads in each.  Poultry was kept in one place in contiguous hutches.  First group was control one and was given ration according to passed in estation scheme.   Experimental group was given ration with added stimular at 1g/head during 15 days.

As a result of experiment it was found positive effect of stimular to poultry organisms (table 1).

Table 1 – Trial results of using stimular in broiler chickens ration

groups dw

1 day


5 days


10 days


15 days


20 days


25 days


30 days





Daily dw


saved Spent feed
control 42 g 122 272 501 807 1198 1628 2022 56,6 94,0 1,74
experimental 42 g 122 270 502 808 1206 1636 2095 58,7 100 1,71


From submitted in the table indices we can see, that in the end of the trial period daily chickens gains of experimental group exceed indices of control one for 3,7%.  After using stimular, spent feed was lower than in control group for 1,8%. Besides, in experimental group safety of poultry was 100%, while in control one it was only 94%.

At the end of the trials was checked the biochemical blood composition of chickens. (table 2).

Table 2 – Results of biochemical blood analysis of broiler chickens

Indices Groups
control experimental
Total protein, g/l 27,5±0,53  30,4±0,55**
Calcium, mmol/L 2,21±0,19 2,95±0,20*
Phosphorus, mmol/L 4,96±0,37 3,84±0,41
Cholesterol mmol/L 3,02±0,24 3,01±0,27
Glucose, mmol/L 11,29±0,76 11,50±0,80
Ferrum μ % 75,9±0,94 80,75±0,86
Vitamin А, µM 1,30±0,18 1,88±0,16*
Alkaline phosphatase u/L 3848,1±81,34 3644,5±85,34
AST u/L 201,3±6,38 170,1±7,20*
ALT u/L 51,62±1,35 52,6±2,18

* р<0,05


From submitted in the table data we can see the differences in biochemical blood composition between control and experimental groups.       This applies especially to total protein, calcium, vitamin A and aspartate transaminase.  For example, after using stimular protein level in experimental group exceeds control group indices for 10,5%, calcium – for 33,4%, vitamin A – for 44,6%.  In all cases the difference between control and experimental groups was proved statistically. (р<0,05-0,01).

It should be emphasized also considerable reduction of aspartate transaminase activity (for 15,5%) in comparison with control (р<0,05).

These changes show positive effect of stimular to liver function and metabolism recovery in poultry organisms.

Results of stimular influence over broiler chickens autarcesis indices are presented in Table 3.

Table 3 – Broiler chickens autarcesis indices

Indices groups
control experimental
Bactericidal activity, % 45,24±2,34 56,13±2,33*
Phagocytic activity, % 45,74±2,11 52,22±2,14*
Lysozyme activity, % 17,13±0,74 17,24±0,91
Immunoglobulins, unit 3,21±0,29 3,76±0,22


From submitted in the table indices we can see, that stimular usage causes proved increasing of  blood serum bactericidal activity and pseudoeosinophiles phagocytic activity for 18,9% and 15,6% therefore (р<0,05) in comparison with control indices.

Thus, our trials showed that stimular had growth-stimulating effectiveness, high bioactivity and increased immunological status.


For autarcesis gain and poultry productivity increasing stimular is possible to use as vitamin-enzyme premix. Speciment is to be added into food assuming 1,0 g/hd during the whole poultry raising period.

Contra-indications for the speciment usage weren’t found out.

Список литературы / References

  1. Draganov I. Influence of multienzymatic preparation over broiler chickens metabolism and productivity / I. Draganov, G. Rabadanova // Problem of production animals biology. – 2011. – № 3. – P. 105-113.
  2. Karput I. Immunology and immunopathology of yound stock desorders / I. Karput. – Minsk: Uradzhaj, 1993. – P. 288.
  3. Noskov S. Monitoring of farm livestock biochemical blood composition / S. Noskov, L. Reznichenko, Y. Kharchenko // Achievements of science and technology AIC. – 2011. – № 2 – P. 55-57.
  4. Okolelova T., Kulakova N. and others Feeding-stuffs and enzymes. – Sergiyev Posad, 2001. – P. 112.
  5. Plesovskikh N. The usage of enzymes preparations in wheat-barley feed mixes during raising broiler chickens. Omsk, 1999. – P. 16. 6. Temiraev R. Usage efficiency of enzyme preparation and phospholipids during raising broiler chickens. / R. Temiraev, A. Baeva.

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